Vezatin Regulates Seizures by Controlling AMPAR-Mediated Synaptic Activity

Background: The role of vezatin in epilepsy remains unknown. Therefore, the aim present study was to investigate underlying roles epilepsy. Methods: expression pattern vivo and vitro investigated by immunofluorescence western blotting. Pilocarpine (PILO)-induced epileptic mice were subjected behavioral tests determine regulating seizure activity. Whole-cell patch-clamp recordings obtained via multiple biochemical techniques explore mechanism which regulates activity. Findings: Vezatin increased hippocampal tissues from PILO-induced mice, brain patients with temporal lobe (TLE), a Mg2+ -free medium-induced model (VSM). knockdown suppressed activity mice. Mechanistically, AMPAR-mediated synaptic events downregulated surface AMPAR GluA1 subunit (GluA1). Interestingly, decreased phosphorylation at serine 845 reduced protein kinase A (PKA) phosphorylation; when PKA H-89 (a selective inhibitor phosphorylation) vitro, downregulatory effects on blocked. Interpretation: We showed that is abnormal demonstrated can modulate 845, requires signaling activation, further affecting neuronal transmission (NST). Funding Statement: This supported National Science Foundation China (No. 81901315, No.81901330, No.81771390) Kuanren Talents Program Second Affiliated Hospital Chongqing Medical University kryc-yq-2123), China. Declaration Interests: None authors have conflicts interest. Ethics Approval human complied Declaration Helsinki ethical principles Institutes Health approved Committee Human Research University. All animal experiments performed Animal conducted accordance international guidelines for studies University, Chongqing, China.